International Journal of Scientific Research in Dental and Medical Sciences

International Journal of Scientific Research in Dental and Medical Sciences

Anti-Inflammatory Activity of Purified 36.3 kDa Coleus Aromatics Leaves Protein: An in Vitro Study

Document Type : Original Article

Authors
Santhosh Kumar Nune 1
Alia Nusruth 2
Ramesh Babu Elle 3
Dinesha Ramadas 4
1 Department of Biochemistry, Prathima Relief Institute of Medical Sciences, Hanamkonda, Telangana, India
2 Department of Biochemistry, Adichunchanagiri Institute of Medical Sciences, Adichunchanagiri University, Karnataka, India
3 Department of Anatomy, Shridevi Institutes of Medical Sciences & Research Hospital, Tumkur, Karnataka, India
4 Department of Biochemistry, Adichunchanagiri School of Natural Sciences, Adichunchanagiri University, Karnataka, India
10.30485/ijsrdms.2025.542501.1676
Abstract
Background and aim: Medicinal plants have recently become the focus of intense study for conservation, as documented pharmacological effects support their traditional uses. This study aims to investigate the anti-inflammatory activity of purified 36.3 kDa Coleus aromaticus leaves protein (CALP) using the Human Red Blood Cells (HRBC) membrane stabilization method. Purified 36.3kDa CALP is used for this study.
Material and methods: The HRBC membrane stabilization method was employed to investigate the anti-inflammatory activity of CALP. All data were expressed as mean ± standard deviation of three replicates (n=3).
Results: The anti-inflammatory activity of the purified CALP was concentration-dependent; as the concentration increased, the percentage of protection also increased. The purified CALP shows a maximum anti-inflammatory activity of 65.93% (±3.12) at 500 μg/ml, which is slightly lower than that of the standard drug Diclofenac sodium [80.34% (±3.90)] at 200 μg/ml. The purified Coleus aromaticus leaves protein exhibits a membrane stabilization effect by inhibiting hypotonicity-induced lysis of erythrocyte membrane.

Conclusions: This study concludes that the purified 36.6 kDa CALP exhibits a stronger membrane-stabilizing property than the standard drug Diclofenac sodium and thus has stronger anti-inflammatory activity. Proteins will continue to serve as a reservoir for the development of potent drugs with less severe and life-threatening adverse effects; however, these results need to be confirmed by in vivo anti-inflammatory studies.
Keywords
Coleus
Diclofenac
Erythrocyte Membrane
Inflammation

Subjects

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International Journal of Scientific Research in Dental and Medical Sciences
Volume 7, Issue 3
Summer 2025
Pages 106-109

  • Receive Date 21 July 2025
  • Revise Date 20 August 2025
  • Accept Date 26 August 2025
  • Publish Date 01 September 2025