Effect of Nicotine Smoke on Genitourinary Organs of Male Albino Rats: An Experimental Study

Document Type : Original Article


Department of Anatomy, Government Medical College and Hospital, Jammu University, Jammu and Kashmir, India


Background and aim: The harmful effects of nicotine have been reported on the lungs and cardiovascular system in many studies, but the most unexplored profile of the effect of nicotine has been the genitourinary organs. In the current study, we planned to evaluate the effects of nicotine smoke on one of the important genitourinary organs, i.e., the testes and kidneys.
Materials and methods: The study was conducted on 12 inbred adult Wistar albino rats; 6 animals acting as a control, and the remaining six acting as a test group. The control group animals were given only sterile water, whereas animals of the test group were exposed to smoke produced from the nicotine wrapped in cotton wool in the dose of 6mg/day three times a day for each session of 5 minutes each for five days. Each rat was exposed to the smoke produced due to nicotine separately in a closed inhalational chamber and not in groups. The rats were euthanized after experimentation, and testis and kidneys were removed and subjected further to tissue processing for histological examination.
Results: The histological examination of tissue sections of the test group revealed marked distortion and degeneration of seminiferous tubules and germ cell lineage and distorted and widened interstitial spaces with loss of Leydig cells. On the other hand, the tissue sections of the control group showed normal histological architecture of testes with normal interstitial spaces and Leydig cells. Additionally, the renal sections of the test group showed dilation of urinary space, shrunken and distorted glomeruli. On the contrary, the renal specimens of the control group also demonstrated normal renal architectural patterns.
Conclusion: the results of the present study concluded that even passive smoke exposure has drastic effects on the genitourinary organs, and hence, its use in public places should be checked.


Main Subjects

[1]  Akomolafe OR, Imafidon CE, Olukiran OS, Oladele AA, Akanji BO. Sub-acute administration of lower doses of nicotine caused sex-dependent improvement of renal function in Wistar rats. Toxicology reports. 2017;4:535-42. https://doi.org/10.1016/j.toxrep.2017.10.001.
[2] Oyeyemi WA, Kolawole TA, Shittu ST, Ajah R, Oyeyemi BF. Effects of ascorbic acid on reproductive functions of male Wistar rats exposed to nicotine. Journal of African Association of Physiological Sciences. 2014;2(2):110-6.
[3]  The American cancer society: Health risks of secondhand smoke 2015.
[4]  World Health Organization. Global Adult Tobacco Survey: India 2009-2010. New Delhi, India: Ministry of Health and Family Welfare, Government of India. 2010.
[5]  Australian Drug Foundation Tobacco Facts: Smoking: what are the effects? ; 2012.
[6]  Mishra A, Chaturvedi P, Datta S, Sinukumar S, Joshi P, Garg A. Harmful effects of nicotine. Indian journal of medical and paediatric oncology: official journal of Indian Society of Medical & Paediatric Oncology. 2015;36(1):24-31. doi: 10.4103/0971-5851.151771.
[7]  Jana K, Samanta PK, De DK. Nicotine diminishes testicular gametogenesis, steroidogenesis, and steroidogenic acute regulatory protein expression in adult albino rats: possible influence on pituitary gonadotropins and alteration of testicular antioxidant status. Toxicological Sciences. 2010;116(2):647-59. https://doi.org/10.1093/toxsci/kfq149.
[8]  Oyeyipo IP, Obembe OO, Oladokun OO, Raji Y. Sperm function and fertility profile following nicotine administration in male rats: Protective potentials of Zingiber officinale. International Journal of Green Pharmacy (IJGP). 2014;8(2). DOI: http://dx.doi.org/10.22377/ijgp.v8i2.373.
[9]  Halimi JM, Philippon C, Mimran A. Contrasting renal effects of nicotine in smokers and non-smokers. Nephrology, dialysis, transplantation: official publication of the European Dialysis and Transplant Association-European Renal Association. 1998;13(4):940-4. https://doi.org/10.1093/ndt/13.4.940.
[10] Aydos K, Güven MC, Can B, Ergün A. Nicotine toxicity to the ultrastructure of the testis in rats. BJU international. 2001;88(6):622-6. https://doi.org/10.1046/j.1464-4096.2001.02384.x.
[11] Binsawad HA. The Possible protective role of antioxidants (Selenium. Vitamin E) in reducing smoking effects on Testes and Pancreas of albino rats. Ass. Univ. Bull. Environ. Res.2011; 14(1): 61-76.
[12] Mosadegh M, Hasanzadeh S, Razi M. Nicotine-induced damages in testicular tissue of rats; evidences for bcl-2, p53 and caspase-3 expression. Iranian Journal of Basic Medical Sciences. 2017;20(2):199-208. doi: 10.22038/ijbms.2017.8249.
[13] Autifi AH, Elsagheir AS, Almandouh MA. Effect of nicotine on the testis of adult albino rat and the possible protective effect of vitamin E. Nat Sci. 2017;15(4):13-9. doi:10.7537/marsnsj150417.02.
[14] Gawish AM, Ramadan S, Hassan AM, Issa AM. Morphometrical, Histopathological, and Cytogenetical Ameliorating Effects of Green Tea Extract on Nicotine Toxicity of the Testis of Rats. J Cytol Histol 1: 105. doi: 10.4172/2157-7099.100010 5.
[15] Oyeyipo IP, Raji Y, Emikpe BO, Bolarinwa AF. Effects of oral administration of nicotine on organ weight, serum testosterone level and testicular histology in adult male rats. Nigerian Journal of Physiological Sciences. 2010;25(1):81-6.
[16] Coşkun G, Özgür H, Doran Ş, Polat S. Ameliorating effects of curcumin on nicotine-induced mice testes. Turkish journal of medical sciences. 2016;46(2):549-60.
[17] Noorhafiza R, Majid AM, Hashida NH. Testosterone level and histological features of tualang honey and nicotine treated male rats. Biomedical Research. 2013;24(3):383-8..
[18] Audi SS, Abraham ME, Borker AS. Effect of cigarette smoke on body weight, food intake and reproductive organs in adult albino rats. IndianJournal of Experimental Biology 2006; 44: 562-65.
[19] Reddy S, Londonkar R, Reddy SO, Patil SB. Testicular changes due to graded doses of nicotine in albino mice. Indian journal of physiology and pharmacology. 1998;42:276-80.
[20] Kolawole TA, Oyeyemi WA, Adigwe C, Leko B, Udeh C, Dapper DV. Honey attenuates the detrimental effects of nicotine on testicular functions in nicotine treated wistar rats. Nigerian Journal of Physiological Sciences. 2015;30(1-2):10-6.
[21] Metwally FG, Karam SH, Elshazli EM, Ghaffar AA, Mahmoud AM, Aziz MA. Protective effect of curcumin on nicotine-induced toxicity of liver and kidney in rats. Review of Research Journal. 2015;4(5):1333-42.
[22] Toklu H, Şehirli Ö, Şahin H, Çetinel Ş, Yeğen BC, Şener G. Resveratrol supplementation protects against chronic nicotine-induced oxidative damage and organ dysfunction in the rat urogenital system. Marmara Pharmaceutical Journal. 2010;14(1):29-40. DOI: 10.12991/201014462.
[23] Adedayo AD, Tijani AA, Musa AA, Adeniyi TD. Histological study of smoke extract of Tobacco nicotiana on the heart, liver, lungs, kidney, and testes of male Sprague-Dawley rats. Nigerian medical journal: journal of the Nigeria Medical Association. 2011;52(4):217-222. doi: 10.4103/0300-1652.93791.
[24] Hassan KA, Ahmed MA, Hassanein KM, Waly H. Ameliorating effect of vitamin C and selenium against nicotine induced oxidative stress and changes of p53 expression in pregnant albino rats. Journal of Advanced Veterinary and Animal Research. 2016;3(4):321-31. http://doi.org/10.5455/javar.2016.c168.
[25] Odokuma EI, Adogbeji JE. Histomorphological effects of nicotine on the kidney. Annals of Bioanthropology. 2017;5(1):9-13. DOI: 10.4103/2315-7992.210254.
[26] Mahmoud GS, Amer AS. Protective effects of vitamin C against nicotine-induced oxidative damage of rat liver and kidney. IOSR-JESTFT. 2014;8(12):50-63. DOI: 10.9790/2402-081245063.
[27] Al-Harbi MS, Hamza RZ. Protective Effect of Resveratrol against Spleen Toxicity of Nicotine in Male Rat: Antioxidant and Histopathological Approaches. Toxicology International. 2016;23(1):79-88.
[28] Al-Awaida W, Akash M, Aburubaiha Z, Talib WH, Shehadeh H. Chinese green tea consumption reduces oxidative stress, inflammation and tissues damage in smoke exposed rats. Iranian journal of basic medical sciences. 2014;17(10):740-46.